Abstract:Theaimofthisstudywastoinvestigatetheinvitroandinvivoefﬁcacyandthetissuereactionofanantibioﬁlmcoating composed of xylitol, triclosan, and polyhexamethylene biguanide. The antimicrobial activity was analyzed by a turbidimetric method. Scanning electron microscopy was used to evaluate the antiadherent property of central venous catheter (CVC) fragmentsimpregnatedwithanantibioﬁlmcoating(I-CVC)incomparisonwithnoncoatedCVC(NC-CVC)fragments.Twoinvivo assaysusingsubcutaneousimplantationofNC-CVCandI-CVCfragmentsinthedorsalareaofratswereperformed.Theﬁrstassay comprised hematological and microbiological analysis. The second assay evaluated tissue response by examining the inﬂammatory reactions after 7 and 21 days. The formulation displayed antimicrobial activity against all tested strains. A bioﬁlm disaggregationwithsigniﬁcantreductionofmicroorganism’sadherenceinI-CVCfragmentswasobserved.Invivoantiadherence results demonstrated a reduction of early bioﬁlm formation of Staphylococcus aureus ATCC 25923, mainly in an external surface of the I-CVC, in comparison with the NC-CVC. All animals displayed negative hemoculture. No signiﬁcant tissue reaction was observed, indicating that the antibioﬁlm formulation could be considered biocompatible. The use of I-CVC could decrease the probability of development of localized or systemic infections.